Layer-by-layer films as engineered biomaterial coatings : applications to musculo-skeletal tissue engineering
par- 12 mai 2014
Lieu du séminaire : salle B204
Catherine Picart, LMGP, Grenoble Institute of Technology and CNRS, Grenoble, France. Email : firstname.lastname@example.org
Professor at Grenoble INP and researcher at the LMGP laboratory (Minatec site), I will first present our lab and the IMBM - Interfaces between Materials and Biological Matter - group. We have three major research axes : 1) Materials for biosensors ; 2) Self-organization of proteins at material surfaces ; 3) Control of cell fate by engineered surfaces. I will then focus more particularly on the 3rd axis. In vivo, cells are embedded in a microenvironment that contains several extracellular matrix proteins, growth factors and also mechanical signals from the tissue and from neighboring cells(1). All these biochemical, mechanical and topographical signals are important to orient cell fate. The layer-by-layer -LbL- technique has emerged in the past 15 years has an interesting method to build multifunctional 2D films or to coat 3D porous materials(2). It is highly tunable as several processing parameters can be varied and everything can be performed in water. Besides, it can be combined with microfabrication techniques. In this presentation, I will present our studies on layer-by-layer films made of polysaccharides and polypeptides to mimic some aspects of this microenvironment, especially controlled mechanical properties(3), bioactivity(4) and topographical properties(5 et 6). This new type of coating films offers a unique opportunity to investigate cellular processes in well defined 2D micro-environments, both to answer fundamental biological questions but also for the development of engineered tissues(7).
1. Hynes RO. The extracellular matrix : not just pretty fibrils. Science 2009. 326:1216-9.
2. Boudou T, Crouzier T, Ren K, Blin G, and Picart C. Multiple functionalities of polyelectrolyte multilayer films : new biomedical applications. Adv. Mater. 2010. 22:441-67.
3. Ren K, Crouzier T, Roy C, and Picart C. Polyelectrolyte multilayer films of controlled stiffness modulate myoblast differentiation. Adv. Funct. Mater. 2008. 18:1378-1389.
4. Crouzier T, Fourel L, Boudou T, Albiges-Rizo C, and Picart C. Presentation of BMP-2 from a soft biopolymeric film unveils its activity on cell adhesion and migration. Adv. Mater. 2011. 23:H111-8.
5. Monge C, Ren K, Berton K, Guillot R, Peyrade D, and Picart C. Engineering muscle tissues on microstructured polyelectrolyte multilayer films Tissue Eng. Part A 2012. 18:1664-1676.
6. Almodovar J, Crouzier T, Selimovic S, Boudou T, Khademhosseini A, and Picart C. Gradients of physical and biochemical cues on polyelectrolyte multilayer films generated via microfluidics. Lab Chip 2013. 13:1562-70.
7. Guillot R, Gilde F, Becquart P, Sailhan F, Lapeyrere A, Logeart-Avramoglou D, and Picart C. The stability of BMP loaded polyelectrolyte multilayer coatings on titanium. Biomaterials 2013. 34:5737-46.
Voir en ligne : https://chimie-biologie.ujf-grenobl...
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